活体牛受精卵原核的可视化。

Visualization of pronuclei in living bovine zygotes.

作者信息

Minhas B S, Capehart J S, Bowen M J, Womack J E, McCrady J D, Harms P G, Wagner T E, Kraemer D C

出版信息

Biol Reprod. 1984 Apr;30(3):687-91. doi: 10.1095/biolreprod30.3.687.

DOI:10.1095/biolreprod30.3.687

Abstract

Bovine embryos were surgically collected from the oviducts of superovulated crossbred heifers 48 h postonset of estrus. The one-celled ova were treated with 4'-6'-diamidino-2-phenylindole (DAPI) and observed under ultraviolet light by fluorescence microscopy. Both male and female pronuclei were visualized, identified and subjected to micromanipulation.

摘要

在发情开始后48小时，通过手术从超排杂交小母牛的输卵管中收集牛胚胎。将单细胞卵用4′-6′-二脒基-2-苯基吲哚（DAPI）处理，并在紫外光下通过荧光显微镜观察。可见雄原核和雌原核，对其进行识别并进行显微操作。