Hatten M E
Brain Res. 1984 Apr;315(2):309-13. doi: 10.1016/0165-3806(84)90166-4.
Three types of astroglia appear during cerebellar development--radial glia and Bergmann glia, which are thought to facilitate neuronal migration, and astrocytes, which are thought to compartmentalize mature granule neurons. Cells resembling Bergmann glia and astrocytes have been described in cultures of cerebellar cells harvested from early postnatal cerebellum. In this study, we have used cell-type specific antisera to visualize embryonic forms of cerebellar astroglia and their interaction with embryonic neurons in vitro. When cells were dissociated from mouse cerebellum on the thirteenth embryonic day (E13), 3 forms of cells were stained with antisera raised against purified glial filament protein ( AbGF ), all of which had more elongated processes and less complex shapes than astroglia from postnatal day 7. The vast majority of embryonic cerebellar neurons did not contact these immature forms of astroglia.
在小脑发育过程中会出现三种类型的星形胶质细胞——放射状胶质细胞和伯格曼胶质细胞，它们被认为有助于神经元迁移；还有星形胶质细胞，被认为能将成熟的颗粒神经元分隔开来。在从出生后早期小脑收获的小脑细胞培养物中，已经描述了类似伯格曼胶质细胞和星形胶质细胞的细胞。在本研究中，我们使用细胞类型特异性抗血清来可视化小脑星形胶质细胞的胚胎形式及其在体外与胚胎神经元的相互作用。当在胚胎第13天（E13）从小鼠小脑中分离细胞时，3种细胞形式被针对纯化的胶质纤维酸性蛋白（AbGF）产生的抗血清染色，所有这些细胞的突起都比出生后第7天的星形胶质细胞更长，形状也更简单。绝大多数胚胎小脑神经元并未与这些未成熟形式的星形胶质细胞接触。
