Reinis S, Goldman J M
Brain Res Bull. 1984 Mar;12(3):339-42. doi: 10.1016/0361-9230(84)90063-7.
Following the intraocular injection of tritiated proline in the mouse, the progressive transport of radioactivity in the brain and the nature of the cortical material(s) to which the label is bound was examined. About 35-40% of the radioactivity that was present in the cerebral cortex at four weeks post-injection was extractable with either distilled water or various buffers. By using 0.1% SDS this value can be increased up to 94%. Polyacrylamide gel electrophoresis of the extracted proteins showed that a major part of the radioactivity is accumulated in one band of proteins. This heavily labeled band could not be identified in the homolateral parietooccipital cortex or in the frontal cortex. Similarly, SDS electrophoresis of the SDS-extracted proteins also demonstrated the presence of a major band of proteins whose molecular weight was estimated at approximately 68,000 daltons. The protein was purified by ammonium sulfate precipitation and DEAE-Sephadex separation.
给小鼠眼内注射氚标记的脯氨酸后，检测了放射性物质在脑中的逐步运输以及与标记物结合的皮质物质的性质。注射后四周时，大脑皮质中约35%-40%的放射性物质可用蒸馏水或各种缓冲液提取。使用0.1%十二烷基硫酸钠（SDS）时，该值可提高至94%。对提取的蛋白质进行聚丙烯酰胺凝胶电泳显示，大部分放射性物质积聚在一条蛋白质带中。在同侧顶枕叶皮质或额叶皮质中无法识别这条高度标记的带。同样，对SDS提取的蛋白质进行SDS电泳也显示存在一条主要蛋白质带，其分子量估计约为68000道尔顿。该蛋白质通过硫酸铵沉淀和二乙氨基乙基-葡聚糖凝胶（DEAE-Sephadex）分离进行纯化。
