Lynch J A, Binnington B D, Hoover D M
Can J Comp Med. 1984 Apr;48(2):233-5.
An outbreak of central nervous system disease affecting young pigs occurred in the fall of 1981 in eastern Ontario. A diagnosis of viral encephalomyelitis was made on pathological grounds and virus isolation studies were subsequently initiated to determine the causative agent. Cultural isolation procedures using several biological systems failed to detect virus in nervous tissues from affected animals. Direct extraction of similar tissues by combined biochemical and biophysical procedures yielded nonenveloped , spherical particles with a diameter of 30 nm and a buoyant density of 1.34 g/mL in CsCl. A tentative diagnosis of enterovirus infection was made on this basis.
1981年秋季，安大略省东部出现了一起影响幼猪的中枢神经系统疾病疫情。基于病理学依据做出了病毒性脑脊髓炎的诊断，随后开展了病毒分离研究以确定病原体。使用多种生物系统的培养分离程序未能在患病动物的神经组织中检测到病毒。通过生化和生物物理联合程序直接提取类似组织，得到了直径为30纳米、在氯化铯中的浮力密度为1.34克/毫升的无包膜球形颗粒。据此做出了肠道病毒感染的初步诊断。
