Sacks P G, Wansor K J, Parsons D F
Cancer Res. 1984 Jul;44(7):3063-74.
An invasion model designed specifically for studying mechanisms of invasion of squamous-cell carcinomas was developed with murine buccal mucosa as the host tissue. The mucosal explants were destratified by growth in low-calcium medium (less than 0.07 mM), which results in a dorsal surface composed of one or two layers of basal epithelial cells. The explant has a three-dimensional histoarchitecture similar to in vivo mucosa. A spontaneously transformed epithelial cell line (Pam 27; Yuspa , S. H., Hawley -Nelson, P., Koehler , B., and Stanley, J. R. Cancer Res., 40: 4694-4703, 1980) was used to seed explants. Attachment and subsequent growth and invasion were monitored. The morphology of attachment was examined by conventional and high-voltage electron microscopy. In addition, attachment was quantitated by using [125I]iododeoxyuridine-labeled tumor cells. Attachment was shown to be an active process which involves an interdigitation of tumor-host cell processes. Junctional complexes were also observed between tumor and host epithelial cells. By 24 hr, tumor cells were spread on the basal cells and were in the process of replacing host cells. Long-term growth of explants showed that tumor cells can repopulate the epithelial surface and invade the stromal region.
以鼠颊黏膜作为宿主组织，开发了一种专门用于研究鳞状细胞癌侵袭机制的侵袭模型。通过在低钙培养基（低于0.07 mM）中培养使黏膜外植体去分层，这会导致背侧表面由一层或两层基底上皮细胞组成。该外植体具有与体内黏膜相似的三维组织结构。使用一种自发转化的上皮细胞系（Pam 27；尤斯帕，S. H.，霍利 - 尼尔森，P.，克勒，B.，以及斯坦利，J. R.《癌症研究》，40: 4694 - 4703，1980）来接种外植体。监测细胞附着以及随后的生长和侵袭情况。通过传统电子显微镜和高压电子显微镜检查附着的形态。此外，使用[125I]碘脱氧尿苷标记的肿瘤细胞对附着进行定量分析。结果表明附着是一个活跃的过程，涉及肿瘤 - 宿主细胞突起的相互交错。在肿瘤与宿主上皮细胞之间也观察到了连接复合体。到24小时时，肿瘤细胞已扩散到基底细胞上，并正在取代宿主细胞。外植体的长期生长表明肿瘤细胞能够重新填充上皮表面并侵入基质区域。
