Coated vesicles of liver Golgi apparatus increased by ascorbate.

Cultured hepatocytes and hepatoma cells when treated for 10-30 min with 30 mM ascorbate (vitamin C) responded by a 2- to 3- fold increase in the numbers of coated vesicles of the Golgi apparatus zone as determined by quantitation from electron micrographs. At a near optimal concentration of 30 mM ascorbate, the effect was reproduced under a variety of conditions of fixation and methods of specimen preparation.