通过皮下注射二硝基芘在大鼠中诱发肉瘤。

Induction of sarcomas in rats by subcutaneous injection of dinitropyrenes.

作者信息

Ohgaki H, Negishi C, Wakabayashi K, Kusama K, Sato S, Sugimura T

出版信息

Carcinogenesis. 1984 May;5(5):583-5. doi: 10.1093/carcin/5.5.583.

DOI:10.1093/carcin/5.5.583

Abstract

The carcinogenicities of potent mutagenic dinitropyrenes were examined by their subcutaneous injections into male F344/ DuCrj rats. Animals were injected with 0.2 mg each of 1,3- and 1,8-dinitropyrenes (1,3- and 1,8- DNPs ) dissolved in 0.2 ml of dimethyl sulfoxide (DMSO) twice a week for 10 weeks. In rats treated with 1,8-DNP, sarcomas were induced at the sites of injection in all 10 animals tested between the 113th and 127th experimental days. In rats treated with 1,3-DNP, 10 of 10 rats developed sarcomas between days 119 and 320. Some tumors induced by these chemicals were transplantable into the same strain of rats. No tumors developed in control rats, which received only DMSO.

摘要

通过将强效诱变剂二硝基芘皮下注射到雄性F344/DuCrj大鼠体内，研究了其致癌性。动物每周两次皮下注射溶解于0.2 ml二甲基亚砜（DMSO）中的0.2 mg 1,3-二硝基芘和1,8-二硝基芘（1,3-DNP和1,8-DNP），持续10周。在接受1,8-DNP治疗的大鼠中，在第113天至第127天之间测试的所有10只动物的注射部位均诱发了肉瘤。在接受1,3-DNP治疗的大鼠中，10只大鼠中有10只在第119天至320天之间发生了肉瘤。这些化学物质诱导的一些肿瘤可移植到同品系大鼠体内。仅接受DMSO的对照大鼠未发生肿瘤。