Norrgren G, Ebendal T, Wikström H
Exp Cell Res. 1984 Jun;152(2):427-35. doi: 10.1016/0014-4827(84)90643-8.
Medium conditioned by embryonic chick heart cells is known to support extensive neurite outgrowth from autonomic and sensory neurons. In the present report we describe the use of microcarrier cell culture with serum-free media to scale up the production of the nerve growth-stimulating factors. A growth medium composed of DME /F10 supplemented with insulin, transferrin, human serum albumin and fibronectin in combination with a low molecular weight (MW) fraction of fetal calf serum (FCS) or a mixture of FGF, dexamethasone, calmodulin and thrombin supported the heart cell proliferation at a rate similar to that of medium with 10% FCS. Furthermore, the level of successively accumulated nerve growth activity measured in a bioassay with sympathetic ganglia proved to be nearly equivalent to what was obtained when cells were grown in medium containing serum. The results confirm the potential of microcarrier cell culture in serum-free media for the production and subsequent recovery of a specific cell product.
已知经胚胎鸡心脏细胞调节的培养基能支持自主神经和感觉神经元长出大量神经突。在本报告中，我们描述了使用微载体细胞培养和无血清培养基来扩大神经生长刺激因子的生产。一种由DME/F10组成的生长培养基，添加胰岛素、转铁蛋白、人血清白蛋白和纤连蛋白，再结合低分子量的胎牛血清（FCS）组分或成纤维细胞生长因子（FGF）、地塞米松、钙调蛋白和凝血酶的混合物，其支持心脏细胞增殖的速率与含10% FCS的培养基相似。此外，在交感神经节生物测定中测得的连续积累的神经生长活性水平，被证明几乎等同于在含血清培养基中培养细胞时所获得的水平。这些结果证实了在无血清培养基中进行微载体细胞培养用于生产和后续回收特定细胞产物的潜力。
