Pack R J, Williams I P, Phipps R J, Richardson P S, Rich B
Eur J Respir Dis. 1984 May;65(4):239-50.
Bronchi, not involved in tumor, were removed from resected human lungs. They were mounted in Ussing chambers containing Krebs-Henseleit solution with radiolabelled precursors of mucus. Airway was examined by both light and electron microscopy. Tissues recently removed from resected lungs exhibited both intra- and extra-cellular oedema and hypoxic changes in mitochondria, but tight junctions between epithelial cells appeared intact, indicating an effective epithelial barrier. Tissues removed from Ussing chambers after up to 6 h showed little oedema and their mitochondria were normal. Their appearance had returned to that of specimens taken that biopsy and fixed immediately. Autoradiographs of specimens from Ussing chambers showed that the mucus-producing cells had concentrated the radiolabels . Mucus in gland ducts was also radiolabelled. Damaged areas of mucous membrane were rare and probably pre-operative. We conclude that resected bronchi, suspended in Ussing chambers, have a structure and secretory activity suitable for investigations of the control of secretion.
从切除的人肺中取出未受肿瘤累及的支气管。将其置于含有带放射性标记的黏液前体的克氏-亨氏溶液的尤斯灌流小室中。通过光学显微镜和电子显微镜检查气道。刚从切除的肺中取出的组织表现出细胞内和细胞外水肿以及线粒体的缺氧变化，但上皮细胞之间的紧密连接似乎完整，表明上皮屏障有效。在长达6小时后从尤斯灌流小室中取出的组织显示几乎没有水肿，其线粒体正常。它们的外观已恢复到活检时立即固定的标本的外观。来自尤斯灌流小室标本的放射自显影片显示，产生黏液的细胞已浓缩了放射性标记物。腺管中的黏液也有放射性标记。黏膜受损区域很少见，可能是术前就有的。我们得出结论，悬浮在尤斯灌流小室中的切除支气管具有适合研究分泌控制的结构和分泌活性。
