Doy T G, Hughes D L
Exp Parasitol. 1984 Jun;57(3):274-8. doi: 10.1016/0014-4894(84)90101-2.
The effective site of resistance to reinfection of cattle with Fasciola hepatica was examined by recovery of challenge flukes from either the liver or body cavity. Calves infected 18 or 26 weeks previously with F. hepatica showed levels of resistance to reinfection of 56 and 94%, respectively, as assessed by recovery of flukes from the liver 15-16 weeks after challenge. Plasma glutamate dehydrogenase (EC 1.4.1.3; GLDH) enzyme activity estimations revealed only a marginal increase in these latter resistant calves compared with previously naive controls, indicating minimal liver damage as a result of migrating flukes. By comparison, when immature challenge flukes were recovered from the body cavity 4 or 14 days after infection of corresponding previously infected or naive calves, there was no significant difference in numbers. It appears, therefore, that, in cattle, resistance mechanisms are effective against challenge flukes at or soon after penetration of the liver.
通过从肝脏或体腔中回收攻击虫体，研究了牛对肝片吸虫再感染产生抗性的有效部位。18周或26周前感染肝片吸虫的犊牛，在攻击后15 - 16周，通过从肝脏中回收虫体评估，对再感染的抗性水平分别为56%和94%。血浆谷氨酸脱氢酶（EC 1.4.1.3；GLDH）酶活性测定显示，与之前未感染的对照相比，这些抗性较强的犊牛仅略有增加，表明迁移的虫体对肝脏造成的损伤最小。相比之下，当在相应的先前感染或未感染的犊牛感染后4天或14天从体腔中回收未成熟的攻击虫体时，数量没有显著差异。因此，在牛中，抗性机制在肝片吸虫穿透肝脏时或之后不久对攻击虫体是有效的。
