Guyton J R, Dao D T, Lindsay K L
Exp Mol Pathol. 1984 Jun;40(3):340-8. doi: 10.1016/0014-4800(84)90051-0.
A new technique for the selective removal of endothelium in the rat carotid artery has been developed, and subsequent events in the vascular wall have been examined. To achieve de-endothelialization, more than 2,000 bubbles of nitrogen in phosphate-buffered saline are passed through a temporarily isolated segment of rat carotid over a period of 3 min. Bubbles are generated by a simple apparatus, consisting of a pressurized tilting chamber and catheter. Endothelium is removed while subendothelial basement membrane and other subjacent structures remain intact. Platelets attach to the denuded surface within minutes after re-establishment of blood flow. Myointimal thickening is found at 5 weeks and 4 months after de-endothelialization. The method is quite reliable and will facilitate further studies of reactions to carotid endothelial injury in young adult rats.
已开发出一种用于选择性去除大鼠颈动脉内皮的新技术，并对血管壁随后发生的事件进行了研究。为实现去内皮化，在3分钟内将2000多个磷酸盐缓冲盐水中的氮气气泡通过大鼠颈动脉的一个临时隔离段。气泡由一个简单的装置产生，该装置由一个加压倾斜室和导管组成。内皮被去除，而内皮下基底膜和其他相邻结构保持完整。在恢复血流后的几分钟内，血小板附着在裸露的表面。去内皮化后5周和4个月发现肌内膜增厚。该方法相当可靠，将有助于对年轻成年大鼠颈动脉内皮损伤反应的进一步研究。
