van der Vliet P C, van Dam D, Kwant M M
FEBS Lett. 1984 Jun 4;171(1):5-8. doi: 10.1016/0014-5793(84)80449-4.
Adenovirus DNA replication was studied in a partially reconstituted system consisting of purified viral proteins (DNA-binding protein, precursor terminal protein and Ad DNA polymerase) and a nuclear extract from uninfected HeLa cells. Optimal DNA replication required the presence of a heat-stable, ribonuclease-sensitive fraction from the cytosol of uninfected cells. This fraction stimulated the initiation about 3-fold and the replication of origin fragments 5-10-fold. Sedimentation analysis indicated the presence of a fast-sedimenting and a slow-sedimenting component which complemented each other. At least part of the stimulation was caused by low-molecular-mass RNA.
在一个部分重构的系统中研究了腺病毒DNA复制，该系统由纯化的病毒蛋白（DNA结合蛋白、前体末端蛋白和腺病毒DNA聚合酶）以及来自未感染的HeLa细胞的核提取物组成。最佳的DNA复制需要存在来自未感染细胞胞质溶胶的热稳定、核糖核酸酶敏感部分。该部分将起始反应刺激约3倍，将起始片段的复制刺激5至10倍。沉降分析表明存在相互补充的快速沉降和慢速沉降成分。至少部分刺激是由低分子量RNA引起的。
