Schnarr M, Daune M
FEBS Lett. 1984 Jun 11;171(2):207-10. doi: 10.1016/0014-5793(84)80489-5.
The interaction of the lexA repressor of E. coli with poly[d(A-T)] has been studied by circular dichroism. The binding induces an about 2-fold increase of the circular dichroism intensity at 263 nm, pointing out a conformational change of the nucleic acid. The observed spectral changes are very similar to those observed for the binding of the lac repressor to poly[d(A-T)] and natural DNA. At elevated ionic strength the binding isotherms do show a pronounced sigmoidal shape indicating a cooperative mode of binding.
通过圆二色性研究了大肠杆菌LexA阻遏物与聚[d(A-T)]的相互作用。这种结合导致263nm处圆二色性强度增加约2倍，表明核酸发生了构象变化。观察到的光谱变化与乳糖阻遏物与聚[d(A-T)]和天然DNA结合时观察到的变化非常相似。在较高离子强度下，结合等温线确实呈现出明显的S形，表明存在协同结合模式。
