Hardison W G, Grundy S M
Gastroenterology. 1984 Jul;87(1):130-5.
Six male subjects with normal gastroenterologic function were studied to determine the effects of ursodeoxycholic (15 mg/kg X day) and tauroursodeoxycholic (20 mg/kg X day) acid feeding on bile acid synthesis and cholesterol absorption. Each bile acid was fed for 1 mo and withheld for the next month. Subjects remained on a metabolic ward and consumed a constant diet of 500 mg of cholesterol mixed with solid and liquid formulas. Before the study started, each subject received 50 muCi of [4-14C]cholesterol intravenously. During the study, stools were collected for the determination of 24-h fecal acidic and neutral sterol excretion, and blood was drawn twice weekly for determination of serum cholesterol specific activity. At the end of each month an intestinal perfusion study was performed to measure total bile acid pool size and hourly biliary secretion rates of cholesterol, phospholipid, and bile acid. From these data, the percentage of cholesterol absorption and total endogenous bile acid synthesis could be calculated. Neither ursodeoxycholic nor tauroursodeoxycholic acid feeding decreased endogenous bile acid synthesis. During bile acid feeding periods, the percentage of cholesterol absorption was decreased.
对六名胃肠功能正常的男性受试者进行了研究，以确定熊去氧胆酸（15毫克/千克×天）和牛磺熊去氧胆酸（20毫克/千克×天）喂养对胆汁酸合成和胆固醇吸收的影响。每种胆汁酸喂养1个月，下个月停用。受试者留在代谢病房，食用含有500毫克胆固醇的固定饮食，该饮食与固体和液体配方混合。在研究开始前，每位受试者静脉注射50微居里的[4-14C]胆固醇。在研究期间，收集粪便以测定24小时粪便酸性和中性固醇排泄量，每周两次采集血液以测定血清胆固醇比活性。在每个月末进行肠道灌注研究，以测量总胆汁酸池大小以及胆固醇、磷脂和胆汁酸的每小时胆汁分泌率。根据这些数据，可以计算出胆固醇吸收百分比和内源性胆汁酸总合成量。熊去氧胆酸和牛磺熊去氧胆酸喂养均未降低内源性胆汁酸合成。在胆汁酸喂养期间，胆固醇吸收百分比降低。
