Ankel E G, Zirneski J, Ring B J, Holcenberg J S
In Vitro. 1984 May;20(5):376-84. doi: 10.1007/BF02619582.
High concentrations of Escherichia coli asparaginase (80 U/ml) altered the binding of concanavalin A (Con A) to L 5178Y murine lymphoma cells that are sensitive to the cytotoxic action of this enzyme. Incubation of the asparaginase sensitive line in asparagine-free media or media containing Acinetobacter glutaminase-asparaginase did not alter the Con A binding of these cells. Escherichia coli asparaginase had no effect on Con A binding of two asparaginase resistant L5178Y cell lines that were isolated and maintained in asparagine depleted or asparaginase containing medium. The E. coli asparaginase preparation inhibited protein and glycoprotein biosynthesis to comparable degrees. It did not have proteolytic or glycolytic activity. Escherichia coli asparaginase did not alter the binding of wheat germ, soybean or ricin agglutinins to any of these cell lines. These data suggest that high concentrations of E. coli asparaginase have a specific effect on the Con A receptor in the sensitive line.
高浓度的大肠杆菌天冬酰胺酶（80 U/ml）改变了刀豆球蛋白A（Con A）与对该酶细胞毒性作用敏感的L 5178Y小鼠淋巴瘤细胞的结合。将天冬酰胺酶敏感细胞系在无天冬酰胺的培养基或含有谷氨酸不动杆菌谷氨酰胺酶-天冬酰胺酶的培养基中培养，不会改变这些细胞的Con A结合。大肠杆菌天冬酰胺酶对两种在天冬酰胺耗尽或含天冬酰胺酶的培养基中分离并维持的抗天冬酰胺酶L5178Y细胞系的Con A结合没有影响。大肠杆菌天冬酰胺酶制剂对蛋白质和糖蛋白生物合成的抑制程度相当。它没有蛋白水解或糖酵解活性。大肠杆菌天冬酰胺酶不会改变小麦胚芽、大豆或蓖麻凝集素与任何这些细胞系的结合。这些数据表明，高浓度的大肠杆菌天冬酰胺酶对敏感细胞系中的Con A受体有特异性作用。
