Sribney M, Dove J L
Lipids. 1978 Jun;13(6):422-6. doi: 10.1007/BF02533712.
The synthesis of acyl-S-pantetheine was found to occur in rat liver microsomal preparations. The reaction required ATP and a metal ion as cofactors, a fatty acid and the reduced form of pantetheine for optimal activity. The Km for pantetheine was 0.8 mM, for ATP 0.8 mM, and for oleic acid 0.3 mM. Mg2+ (20mM), Mn2+ (5 mM), Ca2+ (5mM), and Fe2+ (5 mM) produced approximately equal activity when all other conditions were optimal. The characterization of the product and other properties of the enzyme are described. The acyl-S-pantetheine formed does not act as an acyl donor in the acylation of sn-glycerol-3-phosphate, 1,2-diacylglycerol, or lysolecithin.
酰基-S-泛酰巯基乙胺的合成在大鼠肝脏微粒体制剂中被发现。该反应需要ATP和一种金属离子作为辅助因子、一种脂肪酸以及还原型泛酰巯基乙胺以达到最佳活性。泛酰巯基乙胺的米氏常数为0.8 mM，ATP为0.8 mM，油酸为0.3 mM。当所有其他条件最佳时，Mg2+（20 mM）、Mn2+（5 mM）、Ca2+（5 mM）和Fe2+（5 mM）产生的活性大致相等。描述了产物的特性及该酶的其他性质。所形成的酰基-S-泛酰巯基乙胺在sn-甘油-3-磷酸、1,2-二酰基甘油或溶血卵磷脂的酰化反应中不作为酰基供体。
