Gerson T, Wong M N
Lipids. 1978 Jun;13(6):446-50. doi: 10.1007/BF02533716.
Two groups of male Wistar rats were fed normal (i.e., 18%) and protein-free diets, respectively, for 7 weeks. In vivo incorporation of [1-14C] acetate into palmitic, stearic, oleic, and arachidonic acids by the liver was reduced in the protein-deficient rats. In vitro incubation of liver microsomes with labeled palmitate or linoleate revealed no change in the specific activities of chain elongating or desaturating enzymes. Protein deficiency resulted in a decrease in specific activity of short chain acyl-CoA synthetase and in total CoA, accompanied by the virtual disappearance of acyl-CoA and an increase in free CoA. Furthermore, there was less microsomal fatty acid synthetase and mitochondrial beta-hydroxybutyrate dehydrogenase activity. These results are discussed in relation to fatty acid synthesis and the changes in liver fatty acid composition.
将两组雄性Wistar大鼠分别喂食正常（即18%）和无蛋白饮食7周。蛋白质缺乏的大鼠肝脏中，[1-¹⁴C]乙酸盐在体内掺入棕榈酸、硬脂酸、油酸和花生四烯酸的过程减少。用标记的棕榈酸或亚油酸对肝脏微粒体进行体外孵育，结果显示链延长酶或去饱和酶的比活性没有变化。蛋白质缺乏导致短链酰基辅酶A合成酶的比活性和总辅酶A减少，同时酰基辅酶A几乎消失，游离辅酶A增加。此外，微粒体脂肪酸合成酶和线粒体β-羟基丁酸脱氢酶的活性较低。结合脂肪酸合成以及肝脏脂肪酸组成的变化对这些结果进行了讨论。
