一种对单个淋巴细胞微培养物进行选择性β射线照射的方法及其在辐射敏感性初步研究中的应用。
A method for the selective beta-irradiation of individual lymphocyte microcultures and its application in a preliminary study of radiation sensitivity.
作者信息
Webb C, Sheldon P
出版信息
J Immunol Methods. 1984 Jun 8;71(1):49-59. doi: 10.1016/0022-1759(84)90205-9.
A method that permits high energy beta-irradiation to be applied to individual lymphocyte microplate cultures is described. The principle involves the introduction of single energy sources (in this case 32P) contained within tubes which rest inside wells containing the lymphocyte cultures. Cell viability and mitogen-induced transformation were studied under these conditions. Dose-response effects were clearly demonstrated. The method is simple and, provided the precautions described are adhered to, safe. It should be applicable to studies of radiation sensitivity of putative target cells in various clinical situations.
本文描述了一种允许对单个淋巴细胞微孔板培养物进行高能β射线照射的方法。其原理是将包含单一能量源（在本案例中为32P）的管子引入到装有淋巴细胞培养物的孔内。在这些条件下研究了细胞活力和丝裂原诱导的转化。明确证实了剂量反应效应。该方法简单，并且只要遵守所述的预防措施就是安全的。它应该适用于各种临床情况下推定靶细胞辐射敏感性的研究。
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