脑内乙酰胆碱周转率的测定：一种简单的用于胆碱和乙酰胆碱的高效液相色谱法的辅助方法。

Measurement of acetylcholine turnover rate in brain: an adjunct to a simple HPLC method for choline and acetylcholine.

作者信息

Potter P E, Hadjiconstantinou M, Meek J L, Neff N H

出版信息

J Neurochem. 1984 Jul;43(1):288-90. doi: 10.1111/j.1471-4159.1984.tb06712.x.

DOI:10.1111/j.1471-4159.1984.tb06712.x

Abstract

An existing method for measuring acetylcholine (ACh) and choline (Ch) is shown to be useful for measuring the turnover rate of ACh in mouse brain. Methyl-[3H]Ch is injected into mice. They are killed at different times by microwave irradiation and Ch and ACh extracted and separated by reverse-phase HPLC. Ch and ACh are converted to hydrogen peroxide by a post-column enzyme reaction. Hydrogen peroxide, which is directly related to the tissue content of Ch or ACh, is determined electrochemically. The fractions that correspond to the detector response for Ch and ACh are collected for the measurement of radioactivity. In this way specific radioactivities of endogenous Ch and ACh are estimated in the same sample. We used the specific radioactivity values determined by this procedure to estimate the turnover of ACh for striatum, cerebral cortex, and hippocampus of the mouse.

摘要

一种现有的测量乙酰胆碱（ACh）和胆碱（Ch）的方法被证明可用于测量小鼠脑中ACh的周转率。将甲基-[3H]胆碱注入小鼠体内。在不同时间通过微波辐射将它们处死，然后提取Ch和ACh，并通过反相高效液相色谱法进行分离。Ch和ACh通过柱后酶反应转化为过氧化氢。与Ch或ACh的组织含量直接相关的过氧化氢通过电化学方法测定。收集与Ch和ACh的检测器响应相对应的馏分以测量放射性。通过这种方式，可以在同一样品中估计内源性Ch和ACh的比放射性。我们使用通过该程序确定的比放射性值来估计小鼠纹状体、大脑皮层和海马体中ACh的周转率。