Memo M, Hanbauer I
Neuropharmacology. 1984 Apr;23(4):449-55. doi: 10.1016/0028-3908(84)90254-5.
Prolonged incubation of slices of striatum with agonists of D-1 dopamine receptors increased phosphorylation of at least 5 membrane protein bands. The extent of the increase in phosphate-incorporation depended on the concentration (10(-5) M-10(-4) M) of the agonist in the incubation medium and the duration of incubation (20 min or longer). Preincubation of slices with haloperidol (10(-6) M) greatly reduced, while (-)sulpiride (10(-6) M) failed to alter the increase of phosphorylation elicited by dopamine. Prolonged incubation of striatal slices with LY 141865 (10(-5) M) or isoproterenol (10(-5) M) increased the phosphate-incorporation only in one of the protein bands with an apparent molecular weight of 42,000. Incubation of striatal slices with cholera toxin increased the phosphorylation of protein bands in a similar way to those elicited by dopamine. The present results suggest that the increased phosphorylation of certain protein bands elicited by prolonged exposure of striatal slices to D-1 dopamine receptor agonists may be associated with the desensitization of dopamine-sensitive adenylate cyclase.
将纹状体切片与 D-1 多巴胺受体激动剂长时间孵育，可使至少 5 条膜蛋白带的磷酸化增加。磷酸掺入增加的程度取决于孵育培养基中激动剂的浓度（10⁻⁵ M - 10⁻⁴ M）以及孵育时间（20 分钟或更长）。用氟哌啶醇（10⁻⁶ M）预孵育切片可大大降低磷酸化增加的程度，而（-）舒必利（10⁻⁶ M）未能改变多巴胺引起的磷酸化增加。将纹状体切片与 LY 141865（10⁻⁵ M）或异丙肾上腺素（10⁻⁵ M）长时间孵育，仅使一条表观分子量为 42,000 的蛋白带中的磷酸掺入增加。用霍乱毒素孵育纹状体切片，可使蛋白带的磷酸化增加，其方式与多巴胺引起的相似。目前的结果表明，纹状体切片长时间暴露于 D-1 多巴胺受体激动剂所引起的某些蛋白带磷酸化增加，可能与多巴胺敏感的腺苷酸环化酶脱敏有关。
