Hong D, Stevens P
Pharmacology. 1984;28(5):281-8. doi: 10.1159/000137975.
We investigated the role of protein synthesis in human polymorphonuclear leukocyte (PMN) chemotaxis and luminol-dependent chemiluminescence (CL). We used cycloheximide and puromycin to inhibit protein synthesis, and determined the extent of synthesis by measurement of 14C-amino acid incorporation. With 2-hour incubations both puromycin at 9.0 X 10(-6) M and cycloheximide at 1.8 X 10(-6) M inhibited PMN protein synthesis. At concentrations of 2-4 X 10(-5) M both cycloheximide and puromycin inhibited PMN-chemotaxis 40 and 55%, respectively. However, inhibition was observed only when using zymosan-activated serum and not formyl-methionyl-phenylalanine as a chemoattractant. Using 2-hour incubations, PMN-CL was also suppressed by puromycin and cycloheximide, at 20 and 40%, respectively. The data demonstrated that protein synthesis had an important role in chemotaxis that was dependent on the chemoattractant and perhaps the cellular receptor involved in that process. CL did not require de novo protein synthesis but appeared to depend on protein(s) with a relatively rapid turnover.
我们研究了蛋白质合成在人多形核白细胞（PMN）趋化性和鲁米诺依赖性化学发光（CL）中的作用。我们使用环己酰亚胺和嘌呤霉素抑制蛋白质合成，并通过测量14C-氨基酸掺入来确定合成程度。经过2小时孵育，9.0×10（-6）M的嘌呤霉素和1.8×10（-6）M的环己酰亚胺均抑制PMN蛋白质合成。在2-4×10（-5）M浓度下，环己酰亚胺和嘌呤霉素分别抑制PMN趋化性40%和55%。然而，仅在使用酵母聚糖激活血清作为趋化剂时观察到抑制作用，而使用甲酰甲硫氨酰苯丙氨酸时未观察到抑制作用。经过2小时孵育，PMN-CL也分别被嘌呤霉素和环己酰亚胺抑制20%和40%。数据表明，蛋白质合成在趋化性中具有重要作用，这取决于趋化剂以及该过程中可能涉及的细胞受体。CL不需要从头合成蛋白质，但似乎依赖于周转相对较快的蛋白质。
