针对胸腺嘧啶乙二醇（DNA辐射分解产物）的抗体特性。

Properties of antibodies to thymine glycol, a product of the radiolysis of DNA.

作者信息

Rajagopalan R, Melamede R J, Laspia M F, Erlanger B F, Wallace S S

出版信息

Radiat Res. 1984 Mar;97(3):499-510.

Abstract

Anti-thymine glycol antibodies were elicited by immunizing rabbits with thymine glycol monophosphate (TMP-glycol) conjugated by carbodiimide to BSA. The antibodies produced are specific for thymine glycol as measured by immunoprecipitation of TMP-glycol-RSA conjugates and hapten inhibition of reactivity with OsO4-treated DNA in an enzyme immunoassay. Using the enzyme immunoassay, the antibody is capable of detecting femtomole and picomole levels of thymine glycol in direct and competitive assays, respectively. This immunochemical assay is potentially suitable for measuring the production and repair of thymine glycol damage in cellular DNA.

摘要

通过用经碳二亚胺偶联到牛血清白蛋白（BSA）上的胸腺嘧啶二醇单磷酸酯（TMP-二醇）免疫兔子来产生抗胸腺嘧啶二醇抗体。所产生的抗体对胸腺嘧啶二醇具有特异性，这通过TMP-二醇-兔血清白蛋白（RSA）偶联物的免疫沉淀以及在酶免疫测定中对与经锇酸（OsO4）处理的DNA反应性的半抗原抑制来测定。使用该酶免疫测定，该抗体分别能够在直接测定和竞争性测定中检测飞摩尔和皮摩尔水平的胸腺嘧啶二醇。这种免疫化学测定法可能适用于测量细胞DNA中胸腺嘧啶二醇损伤的产生和修复。

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