Jolesz F A, Polak J F, Ruenzel P W, Adams D F
Radiology. 1984 Jul;152(1):85-7. doi: 10.1148/radiology.152.1.6729140.
Wallerian degeneration of rat sciatic nerves was induced by nerve section. Fifteen days later the degenerated nerves were compared with the intact contralateral nerves from the same animal. Histological sections showed the changes typical of wallerian degeneration: axonal degeneration and secondary demyelination. The freshly dissected nerves were analyzed by magnetic resonance (MR) spectroscopy at 10 MHz, and the water content was determined by dehydration. In the degenerated nerves there was a marked prolongation of both T1 and T2 relaxation times, accompanied by an increase of water content. The results demonstrate the sensitivity of MR for detection of secondary demyelination and disintegration of nerve structures. These results suggest that it should be possible to detect wallerian degeneration in MR images; this will have an important impact on neuropathological diagnosis of central and peripheral nervous system lesions.
通过切断神经诱导大鼠坐骨神经发生华勒氏变性。15天后，将变性神经与同一动物未受损的对侧神经进行比较。组织学切片显示出典型的华勒氏变性变化：轴突变性和继发性脱髓鞘。对新鲜解剖的神经进行10兆赫兹的磁共振（MR）波谱分析，并通过脱水测定水分含量。在变性神经中，T1和T2弛豫时间均显著延长，同时水分含量增加。结果表明MR对检测继发性脱髓鞘和神经结构解体具有敏感性。这些结果提示在MR图像中有可能检测到华勒氏变性；这将对中枢和周围神经系统病变的神经病理学诊断产生重要影响。
