Verschaeve L, Kirsch-Volders M, Hens L, Susanne C
Mutat Res. 1978 Jul;57(3):335-7. doi: 10.1016/0027-5107(78)90218-x.
Peripheral blood lymphocytes of phenyl mercury acetate exposed persons and a control population of the same age were cultured for 48 h. In both populations 100 metaphases were trypsin-banded and caryotyped. The relative position of the metaphase chromosomes was studied by means of centromere--centromere distances (delta2) and centromere--metaphase centre distances (d2) obtained by computer-aided mathematical transformation of the individual metaphase coordinates. By comparing both investigated cell populations we mainly observed that the chromosome combinations which statistically differ in mercury-exposed workers from the controls show an increase of centromere-centromere distances after mercury exposure. From the data we may suggest that phenyl mercury acetate influences at first the position of particular chromosomes; especially D-group chromosomes which are involved in nucleolus organisation. This may be due to a greater density of SH-wearing molecules in that region or to a possible inhibition of specific enzymes regulating the nucleolar activity. The exposure level is however too low to allow definite conclusions in this respect.
对接触醋酸苯汞的人群以及相同年龄的对照人群的外周血淋巴细胞进行了48小时的培养。在这两个人群中，各选取100个中期相进行胰蛋白酶显带和核型分析。通过对各个中期相坐标进行计算机辅助数学变换得到的着丝粒间距离（δ2）和着丝粒与中期相中心距离（d2），研究中期相染色体的相对位置。通过比较两个被研究的细胞群体，我们主要观察到，在接触汞的工人中，与对照组相比有统计学差异的染色体组合在接触汞后着丝粒间距离增加。从这些数据我们可以推测，醋酸苯汞首先影响特定染色体的位置；特别是参与核仁组织的D组染色体。这可能是由于该区域含巯基分子的密度较大，或者是由于调节核仁活性的特定酶可能受到抑制。然而，接触水平过低，无法在这方面得出明确结论。
