Verschaeve L, Driesen M, Kirsch-Volders M, Hens L, Susanne C
Hum Genet. 1979 Jun 19;49(2):147-58. doi: 10.1007/BF00277636.
We studied the chromosome distribution in persons professionally exposed to inorganic lead. The degree of lead exposure was evaluated by biochemical measurements and cytogenetic analysis. The chromosome distribution was analyzed from trypsin banded karyotypes; in particular we studied centromere distances (delta2) and centromere-metaphase-center distances (d2) which were obtained by computer-aided mathematical transformation of the individual metaphase coordinates. Higher concentrations of blood lead and urine delta-ALA and a statistically significant increase in aneuploidy, hypoploidy, and type-B chromosome aberrations revealed appreciable exposure although none of the subjects showed signs of excessive lead absorption. However study of the chromosome distribution showed no major differences with that of the controls indicating that lead acts preferentially (directly or indirectly) on the chromosomes rather than on the spindle apparatus. A dissociation of the acrocentric chromosomes was observed in the lead group when compared with the controls. This is thought to reflect a secondary action of lead on the nucleolar organiser regions.
我们研究了职业性接触无机铅人群的染色体分布情况。通过生化检测和细胞遗传学分析来评估铅暴露程度。从胰蛋白酶显带核型分析染色体分布；具体而言，我们研究了通过对个体中期染色体坐标进行计算机辅助数学转换得到的着丝粒距离（delta2）和着丝粒 - 中期中心距离（d2）。血铅和尿δ-氨基乙酰丙酸浓度较高，以及非整倍体、亚二倍体和B型染色体畸变在统计学上显著增加，表明存在明显暴露，尽管没有受试者表现出铅吸收过量的迹象。然而，染色体分布研究显示与对照组没有重大差异，这表明铅优先（直接或间接）作用于染色体而非纺锤体装置。与对照组相比，在铅暴露组中观察到近端着丝粒染色体的解离。这被认为反映了铅对核仁组织区的次级作用。
