Ferry F, Donner M
Scand J Immunol. 1984 May;19(5):435-45. doi: 10.1111/j.1365-3083.1984.tb00952.x.
The in vitro effects of zinc on natural killer (NK) activity of murine spleen cells were studied. The pretreatment of splenocytes with non-toxic concentrations of ZnSO4 induced a decrease of lytic activity against YAC-1 and RDM4 targets. The lytic function of non-activated and poly(I) X poly(C)-activated NK cells was similarly inhibited. When the interaction of effector cells with zinc was studied for 5 min, a significant inhibition of NK lysis was noted, which was maximal after 30 min. Zinc was undoubtedly responsible for the observed effects, since the concurrent addition of both zinc and suitable concentrations of o-phenanthroline, a Zn++ chelating agent, made it possible to maintain a normal level of lysis. Moreover, the pretreatment of spleen cells with increased concentrations of o-phenanthroline also inhibited NK lysis, suggesting that a physiological intracellular zinc content is required to maintain an optimal lytic function of NK cells. Although the lysis was completely suppressed after the addition of 10(-4) M ZnSO4, the frequency of target-binding cells (TBC), which was assumed to represent the first stage of NK-mediated cytolysis, was only partly inhibited. The results are discussed in view of a possible action of zinc on cell membrane functions, enzyme systems, and release of lymphokines.
研究了锌对小鼠脾细胞自然杀伤（NK）活性的体外作用。用无毒浓度的ZnSO4预处理脾细胞可导致对YAC-1和RDM4靶标的裂解活性降低。未激活的和聚肌苷酸×聚胞苷酸（poly(I) X poly(C)）激活的NK细胞的裂解功能也受到类似抑制。当研究效应细胞与锌的相互作用5分钟时，观察到NK裂解受到显著抑制，30分钟后达到最大抑制。锌无疑是观察到的效应的原因，因为同时添加锌和合适浓度的邻菲罗啉（一种Zn++螯合剂）能够维持正常的裂解水平。此外，用增加浓度的邻菲罗啉预处理脾细胞也抑制了NK裂解，表明需要生理水平的细胞内锌含量来维持NK细胞的最佳裂解功能。尽管在添加10(-4) M ZnSO4后裂解完全被抑制，但假定代表NK介导的细胞溶解第一阶段的靶标结合细胞（TBC）频率仅受到部分抑制。鉴于锌对细胞膜功能、酶系统和淋巴因子释放的可能作用对结果进行了讨论。
