Grignani G, Pacchiarini L, Almasio P, Pagliarino M, Gamba G
Thromb Res. 1984 Apr 15;34(2):147-57. doi: 10.1016/0049-3848(84)90071-9.
In a previous study we found a correlation between metastatic potential and platelet aggregating activity in sublines of a benzopyrene-induced murine fibrosarcoma ( mFS6 ); the purpose of the present work was to elucidate the role of thromboxane biosynthesis by platelets and/or by neoplastic cells in the activation of platelets in this system. The cells of the more malignant subline induced higher aggregation and TxB2 production than those of the non metastasizing one. The supernatants of aggregating cell suspensions contained very few TxB2; furthermore, preincubation of platelets with ASA or Apyrase resulted in inhibition of aggregation and TxB2 production, while preincubation of the cells was ineffective; these results suggest the platelet origin of the measured TxB2 and indicate that platelet-derived ADP plays an important role in their activation, while the production of ADP by the cells does not seem to be relevant in this model. The involvement of platelet prostaglandin biosynthesis pathway in neoplastic cell induced platelet activation could play an important role in the development of platelet-dependent tumour metastasis.
在先前的一项研究中，我们发现苯并芘诱导的小鼠纤维肉瘤（mFS6）亚系中的转移潜能与血小板聚集活性之间存在相关性；本研究的目的是阐明血小板和/或肿瘤细胞中血栓素生物合成在该系统中血小板激活过程中的作用。恶性程度较高的亚系细胞比非转移亚系细胞诱导出更高的聚集和血栓素B2（TxB2）生成。聚集细胞悬液的上清液中TxB2含量极少；此外，血小板与阿司匹林（ASA）或腺苷三磷酸双磷酸酶（Apyrase）预孵育会导致聚集和TxB2生成受到抑制，而细胞预孵育则无效；这些结果表明所测TxB2源自血小板，并表明血小板衍生的二磷酸腺苷（ADP）在其激活过程中起重要作用，而细胞产生的ADP在该模型中似乎无关紧要。血小板前列腺素生物合成途径参与肿瘤细胞诱导的血小板激活可能在血小板依赖性肿瘤转移的发展中起重要作用。
