大鼠大脑中动脉闭塞后脑pH值的放射自显影测定

Autoradiographic determination of brain pH following middle cerebral artery occlusion in the rat.

作者信息

Kobatake K, Sako K, Izawa M, Yamamoto Y L, Hakim A M

出版信息

Stroke. 1984 May-Jun;15(3):540-7. doi: 10.1161/01.str.15.3.540.

DOI:10.1161/01.str.15.3.540

Abstract

A method of quantitative autoradiography using 14C-labelled 5,5-dimethyl-2, 4-oxazolidinedione 14C-DMO to evaluate the local changes in brain pH after ischemia is described. In normal control rats the calculated tissue pH values in gray matter were slightly lower than those in white matter, and there was no significant difference in the calculated pH among the various structures in cortical and subcortical gray matter. Four hours after a left middle cerebral artery (MCA) occlusion, marked reductions in 14C-DMO concentrations were demonstrated in the anterior two-thirds of the cerebral cortex and in the lateral part of the caudate nucleus indicating tissue acidosis in these areas. Although several assumptions are required for the calculation of pH in brain tissue, this method would appear very useful in the investigation of the altered metabolic state in ischemic brain.

摘要

本文描述了一种使用¹⁴C标记的5,5-二甲基-2,4-恶唑烷二酮（¹⁴C-DMO）进行定量放射自显影的方法，以评估缺血后脑局部pH值的变化。在正常对照大鼠中，灰质中计算出的组织pH值略低于白质，皮质和皮质下灰质的不同结构之间计算出的pH值无显著差异。左侧大脑中动脉（MCA）闭塞4小时后，大脑皮质前三分之二和尾状核外侧部分的¹⁴C-DMO浓度显著降低，表明这些区域存在组织酸中毒。尽管计算脑组织pH值需要几个假设，但该方法在研究缺血性脑代谢状态改变方面似乎非常有用。