Suzuki N, Kojima T, Kuwata T, Nishimaki J, Takakubo Y, Miki T
Virology. 1984 May;135(1):20-9. doi: 10.1016/0042-6822(84)90113-2.
Correlation between susceptibility to the anticellular effect of human interferon (HuIFN) and ultraviolet (uv) lethality was examined in a set of isogeneous human cell lines (RSa and IFr cells), a human endometrial cancer cell line (HEC-1 cells), and a Xeroderma pigmentosum-derived fibroblast cell line ( CRL1200 cells). IFr cells, previously established as a HuIFN-alpha-resistant subline by exposing HuIFN-alpha- and uv-sensitive RSa cells to HuIFN-alpha preparations, appeared more refractory to uv than did the parent RSa cells in the cell proliferation and colony-formation studies. The extent of recovery from uv-inhibited total cellular DNA synthesis and uv-induced DNA-repair synthesis was enhanced to a greater extent in IFr cells than in RSa cells. The preexposure of RSa cells to HuIFN-alpha enhanced uv-induced DNA-repair replication activities. HEC-1 cells, which are reportedly totally refractory to HuIFN actions, appeared most resistant to uv, in all the tests. The uv-sensitive CRL1200 cells appeared highly susceptible to HuIFN-beta, in both cell proliferation and DNA-synthesis inhibition tests. These results support and extend our previous finding (N. Suzuki, J. Nishimaki , and T. Kuwata (1982). Mutat . Res. 106, 357-376) that susceptibility to the anticellular effect of HuIFN closely relates with uv lethality.
在一组同基因人类细胞系（RSa和IFr细胞）、一种人子宫内膜癌细胞系（HEC-1细胞）以及一种着色性干皮病来源的成纤维细胞系（CRL1200细胞）中，研究了对人干扰素（HuIFN）抗细胞效应的敏感性与紫外线（uv）致死性之间的相关性。IFr细胞是通过将对HuIFN-α和uv敏感的RSa细胞暴露于HuIFN-α制剂而先前建立的HuIFN-α抗性亚系，在细胞增殖和集落形成研究中，IFr细胞对uv的耐受性似乎比亲代RSa细胞更强。与RSa细胞相比，IFr细胞从uv抑制的总细胞DNA合成和uv诱导的DNA修复合成中恢复的程度更大。RSa细胞预先暴露于HuIFN-α可增强uv诱导的DNA修复复制活性。据报道，对HuIFN作用完全耐受的HEC-1细胞在所有测试中对uv的抗性最强。在细胞增殖和DNA合成抑制测试中，对uv敏感的CRL1200细胞对HuIFN-β高度敏感。这些结果支持并扩展了我们先前的发现（N. Suzuki、J. Nishimaki和T. Kuwata（1982年）。突变研究。106，357 - 376），即对HuIFN抗细胞效应的敏感性与uv致死性密切相关。
