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通过用不含氧载体的培养基进行血管灌注来长期维持内耳蜗电位。

Prolonged maintenance of endocochlear potential by vascular perfusion with media devoid of oxygen carriers.

作者信息

Kobayashi T, Rokugo M, Marcus D C, Comegys T H, Thalmann R

出版信息

Arch Otorhinolaryngol. 1984;239(3):243-7. doi: 10.1007/BF00464250.

DOI:10.1007/BF00464250
PMID:6732601
Abstract

A method is described for maintaining the cochlear potentials of the guinea pig via arterial perfusion of the surviving inner ear with an artificial medium devoid of oxygen carriers or oncotic agents. The endocochlear potential (EP) can be maintained at a normal level for periods in excess of 5 h; the responses of the EP to anoxia and to furosemide closely approximate those seen in the intact animal. This preparation may represent a simplified method for carrying out selected arterial perfusion experiments in the surviving inner ear.

摘要

本文描述了一种通过用不含氧载体或胶体渗透压剂的人工培养基对存活的内耳进行动脉灌注来维持豚鼠耳蜗电位的方法。内淋巴电位(EP)可在正常水平维持超过5小时;EP对缺氧和呋塞米的反应与完整动物中的反应非常相似。这种制备方法可能代表了一种在存活内耳中进行特定动脉灌注实验的简化方法。

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Prolonged maintenance of endocochlear potential by vascular perfusion with media devoid of oxygen carriers.通过用不含氧载体的培养基进行血管灌注来长期维持内耳蜗电位。
Arch Otorhinolaryngol. 1984;239(3):243-7. doi: 10.1007/BF00464250.
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引用本文的文献

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Effects of anoxia on the cochlear summating potential in the guinea pig.
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本文引用的文献

1
Minimal concentrations of metabolic substrates capable of supporting cochlear potentials.
Hear Res. 1982 May;7(1):105-14. doi: 10.1016/0378-5955(82)90084-3.
2
Effect of substrate-free vascular perfusion upon cochlear potentials and glycogen of the stria vascularis.
Hear Res. 1982 Feb;6(2):223-40. doi: 10.1016/0378-5955(82)90056-9.
3
Vascular perfusion of the cochlea: effect of potassium-free and rubidium-substituted media.
Arch Otorhinolaryngol. 1979;225(2):79-81. doi: 10.1007/BF00455206.
4
Maintenance of cochlear function with artificial oxygen carriers.
Laryngoscope. 1979 Sep;89(9 Pt 1):1457-73. doi: 10.1002/lary.5540890911.