Schweiger A, Kostka G
Biochem Biophys Res Commun. 1984 May 16;120(3):996-1001. doi: 10.1016/s0006-291x(84)80205-3.
Nuclear matrix was prepared from unstimulated rat liver by treatment of nuclei with DNAse and 0.4 M NaCl and was further extracted with 2.0 M NaCl. Proteins were bound to poly(rI):(rC)-agarose, incubated with (alpha-32P) adenosine 5'-triphosphate and 2',5'-linked oligoadenylate was isolated from the supernatant. The substance inhibited amino acid incorporation in a reticulocyte translation system and was identified after enzymatic treatment followed by thin-layer chromatography on PEI-cellulose. The possible function of 2',5'-oligo(A) synthetase in the maturation of pre-mRNA associated with nuclear matrix is discussed.
通过用DNA酶和0.4M NaCl处理细胞核，从未受刺激的大鼠肝脏中制备核基质，并用2.0M NaCl进一步提取。蛋白质与聚（rI）：（rC）-琼脂糖结合，与（α-32P）腺苷5'-三磷酸一起孵育，并从上清液中分离出2'，5'-连接的寡腺苷酸。该物质在网织红细胞翻译系统中抑制氨基酸掺入，并在酶处理后通过在PEI-纤维素上的薄层色谱法进行鉴定。讨论了2'，5'-寡腺苷酸合成酶在与核基质相关的前体mRNA成熟中的可能功能。
