Lykkesfeldt A E, Larsen J K, Christensen I J, Briand P
Br J Cancer. 1984 Jun;49(6):717-22. doi: 10.1038/bjc.1984.113.
The effect of 10(-6) M tamoxifen on MCF-7 cells adapted to growth in 0.5% foetal calf serum has been studied. The growth inhibitory effect of this tamoxifen concentration was abolished by simultaneous addition of 10(-8) M oestradiol, indicating that tamoxifen may exert its effect via binding to the oestrogen receptor. Flow cytometric cell cycle analysis of tamoxifen-treated cultures showed an increase in the proportion of cells in the G1 phase of the cell cycle. By exposing the cells to BUdR before flow cytometry the growth fraction was determined and found to be dramatically decreased in tamoxifen-treated cultures. Cells were not only arrested in the G1 phase but also in the G2 phase of the cell cycle. A few colonies of MCF-7 cells were resistant to 10 days treatment with 10(-6) M tamoxifen.
研究了10⁻⁶ M他莫昔芬对适应于在0.5%胎牛血清中生长的MCF-7细胞的作用。同时添加10⁻⁸ M雌二醇可消除该他莫昔芬浓度的生长抑制作用,这表明他莫昔芬可能通过与雌激素受体结合发挥其作用。对经他莫昔芬处理的培养物进行流式细胞术细胞周期分析显示,细胞周期G1期的细胞比例增加。通过在流式细胞术之前将细胞暴露于溴脱氧尿苷来测定生长分数,发现在经他莫昔芬处理的培养物中生长分数显著降低。细胞不仅停滞在细胞周期的G1期,也停滞在G2期。少数MCF-7细胞集落对10⁻⁶ M他莫昔芬处理10天具有抗性。