色素沉着和无色素极端嗜盐菌中的光复活作用。
Photoreactivation in pigmented and non-pigmented extreme halophiles.
作者信息
Sharma N, Hepburn D, Fitt P S
出版信息
Biochim Biophys Acta. 1984 Jun 15;799(2):135-42. doi: 10.1016/0304-4165(84)90287-3.
The sensitivity to ultraviolet radiation (254 nm) and the photoreactivability of four pigmented and three colourless strains of the extremely halophilic bacteria Halobacterium cutirubrum and Halobacterium salinarium have been studied. The results with three pigmented/non-pigmented pairs show that the pigments play an accessory role in photoreactivation at low visible light intensities and confirm that they do not provide passive protection against ultraviolet light. Evidence is presented that photoreactivation plays an unexpected direct role in the resistance of extreme halophiles to ultraviolet radiation and that colourless mutants of H. cutirubrum NRC 34001 only arise in cultures that have been both ultraviolet-irradiated and photoreactivated. None of these extreme halophiles is capable of excision repair of ultraviolet damage to DNA.
对极端嗜盐菌红皮盐杆菌（Halobacterium cutirubrum）和盐沼盐杆菌（Halobacterium salinarium）的四个色素沉着菌株和三个无色菌株对紫外线辐射（254纳米）的敏感性及光复活能力进行了研究。对三对色素沉着/无色菌株的研究结果表明，这些色素在低可见光强度下的光复活过程中起辅助作用，并证实它们不能提供对紫外线的被动保护。有证据表明，光复活在极端嗜盐菌对紫外线辐射的抗性中发挥了意想不到的直接作用，并且红皮盐杆菌NRC 34001的无色突变体仅出现在经过紫外线照射和光复活处理的培养物中。这些极端嗜盐菌均不能对DNA的紫外线损伤进行切除修复。
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