Freyssinet J M, Torbet J, Hudry-Clergeon G
Biochimie. 1984 Feb;66(2):81-5. doi: 10.1016/0300-9084(84)90183-4.
When fibrin polymerizes in a strong magnetic field, it can be highly oriented. The structural diffraction study of the oriented polymer becomes thus possible. The magnetic birefringence can also be used to study the development of the polymer Fibrinogen in solution is weakly oriented in high magnetic fields. In this work we present complementary results and discussion. The validity of the comparison of the orientation parameters of fibrinogen and fibrin with those of other orientable known biological structures is discussed. The orientation of fibrin formed from fibrin monomer solution is compared to that of fibrin formed by the action of thrombin on fibrinogen. The conditions to obtain highly oriented fibrin gels suitable for three dimensional structure studies are also briefly discussed.
当纤维蛋白在强磁场中聚合时，它可以高度取向。因此，对取向聚合物进行结构衍射研究成为可能。磁双折射也可用于研究聚合物的形成。溶液中的纤维蛋白原在强磁场中取向较弱。在这项工作中，我们给出了补充结果和讨论。讨论了将纤维蛋白原和纤维蛋白的取向参数与其他可取向的已知生物结构的取向参数进行比较的有效性。将由纤维蛋白单体溶液形成的纤维蛋白的取向与凝血酶作用于纤维蛋白原形成的纤维蛋白的取向进行了比较。还简要讨论了获得适用于三维结构研究的高度取向纤维蛋白凝胶的条件。
