Crissman R S
Blood Vessels. 1984;21(4):156-70. doi: 10.1159/000158508.
Stereo scanning electron microscopy was used to study the normal arrangement of elastic fibers in canine saphenous veins. Selective alkali digestion and autoclaving exposed a network of interconnecting elastic fibers throughout the venous wall. 14 normal saphenous veins were surgically removed from 7 healthy dogs. Unfixed 1-cm segments of the isolated specimens were digested by sonication for 30 min in 0.5 N NaOH solution and then autoclaved for 30 min. The digested specimens were then fixed and routinely prepared for scanning electron microscopy. Large longitudinally oriented fibers in each tunica branched and anastomosed with adjacent fibers. Small fibers branched off at almost right angles and interconnected the longitudinal fibers. The configuration of the elastic network varied between the tunicae of the venous wall. The different networks were interconnected by anastomosing fibers of adjacent tunica to form a single elastic cylinder throughout the venous wall. The internal elastic lamina consisted of a single layer of fibers with a fishnet-like arrangement around the periphery of the luminal surface. Large, longitudinally oriented elastic fibers of the tunica media branched and anastomosed as in the internal elastic lamina. However, they also had tangential orientation and traversed different levels within the media. As a result, stratified layers were not formed. The external lamina consisted of several parallel layers of longitudinally oriented ribbons of elastic. Thick fibers interconnected these broad ribbons on the same and adjacent levels to form discontinuous layers. The configuration of the elastic network of the canine saphenous vein is compared to that previously reported for human saphenous vein. It is suggested that the architecture of this elastic network would contribute to vascular integrity and flexibility as well as aid in the distribution of stress throughout the venous wall. Basic knowledge of the elastic fiber network in this vein may be applied to future studies of diseased and grafted vessels.
采用立体扫描电子显微镜研究犬隐静脉弹性纤维的正常排列。选择性碱消化和高压灭菌暴露出贯穿静脉壁的相互连接的弹性纤维网络。从7只健康犬身上手术切除14条正常隐静脉。将分离标本未固定的1厘米片段在0.5N氢氧化钠溶液中超声处理30分钟，然后高压灭菌30分钟。然后将消化后的标本固定并常规制备用于扫描电子显微镜观察。每个膜层中粗大的纵向纤维与相邻纤维分支并吻合。细小纤维以几乎直角分支并连接纵向纤维。静脉壁各膜层之间弹性网络的构型各不相同。不同的网络通过相邻膜层的吻合纤维相互连接，在整个静脉壁形成一个单一的弹性圆柱体。内弹性膜由单层纤维组成，在管腔表面周边呈鱼网状排列。中膜粗大的纵向弹性纤维如内弹性膜一样分支并吻合。然而，它们也有切向取向并穿过中膜内的不同层面。结果，未形成分层。外膜由几层平行的纵向弹性带组成。粗大纤维在同一和相邻层面连接这些宽的带，形成不连续的层。将犬隐静脉弹性网络的构型与先前报道的人隐静脉的构型进行比较。提示这种弹性网络的结构有助于血管的完整性和柔韧性，并有助于应力在整个静脉壁的分布。该静脉弹性纤维网络的基础知识可能应用于未来对病变血管和移植血管的研究。
