Hecht S S, Morrison J B, Young R
Carcinogenesis. 1984 Jul;5(7):979-81. doi: 10.1093/carcin/5.7.979.
Urinary metabolites in the rat of the carcinogen N,N-dinitrosopiperazine were identified as N-nitroso(2-hydroxyethyl)-glycine (22% of the dose), N-nitrosodiethanolamine (3%), 3-hydroxy-N-nitrosopyrrolidine (3%), and unchanged N,N-dinitrosopiperazine (18%). N-Nitroso(2-hydroxyethyl)glycine (1.6 mumol) was detected by gas chromatography-nitrosamine specific detection in the urine of rats treated by gavage with 19 mumol of piperazine and 191 mumol of sodium nitrite. Since sensitive methods are available to quantify N-nitroso(2-hydroxyethyl)glycine in rat urine and human urine, it has potential utility as a monitor for in vivo formation of N,N-dinitrosopiperazine.
致癌物N,N-二亚硝基哌嗪在大鼠体内的尿液代谢产物被鉴定为N-亚硝基（2-羟乙基）-甘氨酸（占剂量的22%）、N-亚硝基二乙醇胺（3%）、3-羟基-N-亚硝基吡咯烷（3%）以及未变化的N,N-二亚硝基哌嗪（18%）。通过气相色谱-亚硝胺特异性检测，在经口灌胃给予19 μmol哌嗪和191 μmol亚硝酸钠的大鼠尿液中检测到了1.6 μmol的N-亚硝基（2-羟乙基）甘氨酸。由于有灵敏的方法可对大鼠尿液和人尿液中的N-亚硝基（2-羟乙基）甘氨酸进行定量，它有潜力作为N,N-二亚硝基哌嗪体内形成的监测指标。
