Clark P, Fahy G M, Karow A M
Cryobiology. 1984 Jun;21(3):260-73. doi: 10.1016/0011-2240(84)90322-5.
A renal cortical slice model was used to assess the effects on viability of three vehicle solutions-Krebs-Henseleit (K-H), solution A, and RPS-2--at 25 degrees C. After 120 min incubation no differences in [K+]/[Na+] ratios were found. Tracer techniques were used to study the osmotic effects and permeation kinetics at 25 degrees C of three cryoprotectants (dimethyl sulfoxide (Me2SO), ethylene glycol, and glycerol) and the effect of the vehicle solution (K-H or RPS-2) on Me2SO kinetics. It was found that Me2SO was most permeable and ethylene glycol least, and that ethylene glycol had unusual effects which suggest that it may not act as a simple solute. Differences were found when Me2SO was introduced in K-H and RPS-2 that are believed to be related to the binding properties of Me2SO to cell constituents.
采用肾皮质切片模型评估三种溶媒溶液（克氏-亨氏（K-H）溶液、溶液A和RPS-2）在25℃时对活力的影响。孵育120分钟后，未发现[K⁺]/[Na⁺]比值存在差异。运用示踪技术研究了三种冷冻保护剂（二甲基亚砜（Me₂SO）、乙二醇和甘油）在25℃时的渗透效应和渗透动力学，以及溶媒溶液（K-H或RPS-2）对Me₂SO动力学的影响。结果发现，Me₂SO的渗透性最强，乙二醇的渗透性最弱，且乙二醇具有异常效应，这表明它可能并非作为一种简单溶质起作用。当在K-H和RPS-2中引入Me₂SO时发现了差异，这被认为与Me₂SO与细胞成分的结合特性有关。
