Gadarowski J J, Esce J D
Cryobiology. 1984 Jun;21(3):359-70. doi: 10.1016/0011-2240(84)90331-6.
Acute systemic blood changes were measured in New Zealand white rabbits after severe and mild frostbite injury to the foot. There were observed after 72 hr, in the severely frostbitten rabbits, a decrease in erythrocytes, hematocrit, lymphocytes, and albumin, and an increase in total leukocytes, neutrophils, platelets, fibrinogen, and antithrombin III. Mildly frostbitten rabbits showed similar changes except for no changes in the platelets, albumin, and antithrombin III. In severely frostbitten rabbits, after 72 hr, the changes in the plasma coagulation tests were a prolonged partial thromboplastin time, an accelerated prothrombin time, and increased activities of Factors VII, IX, X, and XI. In mildly frostbitten rabbits there were a prolonged partial thromboplastin time and an increased activity of Factor VII. No changes in fibrinolysis were seen in either group of rabbits. Platelet aggregation, studied only in the severely frostbitten rabbits, showed a change only by an increase in the slope of the collagen-induced platelet aggregation. The blood changes observed in the rabbit model are different than those reported in human frostbite cases. No disseminated intravascular coagulation was apparent in the rabbit model after frostbite injury.
在新西兰白兔足部遭受重度和轻度冻伤后，对其急性全身血液变化进行了测量。在重度冻伤的兔子中，72小时后观察到红细胞、血细胞比容、淋巴细胞和白蛋白减少，而总白细胞、中性粒细胞、血小板、纤维蛋白原和抗凝血酶III增加。轻度冻伤的兔子表现出类似变化，但血小板、白蛋白和抗凝血酶III无变化。在重度冻伤的兔子中，72小时后血浆凝血试验的变化为部分凝血活酶时间延长、凝血酶原时间加快以及因子VII、IX、X和XI的活性增加。在轻度冻伤的兔子中，部分凝血活酶时间延长且因子VII活性增加。两组兔子均未观察到纤维蛋白溶解的变化。仅在重度冻伤的兔子中研究了血小板聚集，结果显示仅在胶原诱导的血小板聚集斜率增加方面有变化。在兔模型中观察到的血液变化与人类冻伤病例报告的不同。冻伤损伤后兔模型中未出现明显的弥散性血管内凝血。
