Bertini I, Luchinat C, Messori L, Scozzafava A
Eur J Biochem. 1984 Jun 1;141(2):375-8. doi: 10.1111/j.1432-1033.1984.tb08201.x.
The bis and mono cobalt(II) derivatives of conalbumin in the presence of bicarbonate have been prepared. The 1H NMR spectra have been recorded at 60 MHz. Well-shaped isotropically shifted signals in the range -100 to +100ppm have been observed for both the mono and bis cobalt(II) derivatives; besides the intensity, all the isotropically shifted signals for the two derivatives are superimposable, indicating that the two sites are so similar as to be indistinguishable within the resolution of the technique. With the aid of T1 measurements, the signals have been assigned to the protons of two histidine and two tyrosinate ligands. The spectra are consistent with six-coordinated, high-spin cobalt(II) chromophores with two trans tyrosinate moieties at each site.
已制备了在碳酸氢盐存在下伴清蛋白的双钴（II）衍生物和单钴（II）衍生物。在60MHz下记录了1H NMR谱。单钴（II）衍生物和双钴（II）衍生物在-100至+100ppm范围内均观察到形状良好的各向同性位移信号；除强度外，两种衍生物的所有各向同性位移信号均可叠加，表明这两个位点非常相似，以至于在该技术的分辨率范围内无法区分。借助T1测量，已将信号归属为两个组氨酸和两个酪氨酸盐配体的质子。这些光谱与每个位点具有两个反式酪氨酸盐部分的六配位、高自旋钴（II）发色团一致。
