一种固定化抗雌激素除了能结合雌激素受体蛋白外，还能结合一种特定的子宫蛋白。

An immobilized antiestrogen binds a specific uterine protein in addition to estrogen receptor proteins.

作者信息

Van Oosbree T R, Olsen M R, Tate A C, Jordan V C, Mueller G C

出版信息

Mol Cell Endocrinol. 1984 May;35(2-3):143-9. doi: 10.1016/0303-7207(84)90010-8.

Abstract

An antiestrogen affinity resin was synthesized by conjugating LY117018, a benzothiophene -derived antiestrogen, to epoxy-activated agarose. This affinity resin bound the Mr = 50 000 and 65 000 estrogen receptor proteins of rabbit uterine cytosol; in addition, it retained a protein from the cytosols of both rat and rabbit uteri that exhibited an ability to interact specifically with LY117018. The possibility that the LY117018 binding protein, which is distinct from estrogen receptors, may play a role in the antiestrogenic actions of LY117018 is discussed.

摘要

通过将一种苯并噻吩衍生的抗雌激素药物LY117018与环氧活化琼脂糖偶联，合成了一种抗雌激素亲和树脂。这种亲和树脂能结合兔子宫胞质溶胶中分子量为50000和65000的雌激素受体蛋白；此外，它还保留了大鼠和兔子宫胞质溶胶中的一种蛋白，该蛋白表现出与LY117018特异性相互作用的能力。文中讨论了与雌激素受体不同的LY117018结合蛋白可能在LY117018的抗雌激素作用中发挥作用的可能性。