Flajnik M F, Horan P K, Cohen N
Dev Biol. 1984 Jul;104(1):247-54. doi: 10.1016/0012-1606(84)90052-6.
Quantitative flow cytometry was used to examine the embryonic origin of lymphocytes in Xenopus laevis. Reciprocal head/body transplants were made between diploid (2N) and triploid (3N) embryos of the same developmental stages ranging from neural plate to tail bud stages. Thymuses and spleens were removed from postmetamorphic chimeras. Cell suspensions were stained with the fluorescent DNA stain, mithramycin, and the ploidy (relative fluorescence intensity) of the cells was then determined by flow cytometry. All lymphocytes in the chimeras were derived from the posterior portion of the embryo. In other experiments, various regions of the lateral plate or ventral mesoderm were grafted from triploid to diploid embryos. Only transplants that included middorsal mesoderm gave rise to lymphocytes.
采用定量流式细胞术研究非洲爪蟾淋巴细胞的胚胎起源。在处于从神经板到尾芽阶段的相同发育时期的二倍体（2N）和三倍体（3N）胚胎之间进行头/体相互移植。从变态后的嵌合体中取出胸腺和脾脏。细胞悬液用荧光DNA染料光辉霉素染色，然后通过流式细胞术测定细胞的倍性（相对荧光强度）。嵌合体中的所有淋巴细胞均来源于胚胎后部。在其他实验中，将侧板或腹侧中胚层的不同区域从三倍体胚胎移植到二倍体胚胎。只有包含中背侧中胚层的移植能产生淋巴细胞。
