昆虫多滋养型卵囊中的钙结合位点作为钙流入途径的可能标志物。
Calcium binding sites in the insect polytrophic egg vesicles as possible markers of the route of inflow of calcium.
作者信息
Przełecka A, Sobota A
出版信息
Folia Histochem Cytobiol. 1984;22(1):43-7.
Calcium binding sites were revealed in the polytropic egg vesicles of Galleria mellonella (Lepidoptera) by fixation of the ovariole with glutaraldehyde in the presence of excess of calcium. Calcium dependent deposits appeared in the mitochondrial matrix in cells of the epithelial sheath and of the follicular epithelium. The deposits were also seen in the extracellular space around the follicular epithelial cells and around the trophocytes , at the oolemma, and in the coated vesicles of the oocytes at the stage of vitellogenesis. On the other hand, the deposits were not detected in the mitochondria of the trophocytes and the oocytes. Considering the high content of calcium in the insect oocyte ( Przel ecka et al. [17]) the possible route of entry of calcium to the oocyte is discussed on the basis of distribution of the visualized calcium binding sites.
通过在过量钙存在的情况下用戊二醛固定卵巢小管，在大蜡螟（鳞翅目）的多态性卵囊泡中发现了钙结合位点。钙依赖性沉积物出现在上皮鞘细胞和卵泡上皮细胞的线粒体基质中。在卵泡上皮细胞周围和滋养细胞周围的细胞外空间、卵膜处以及卵黄发生阶段卵母细胞的被膜小泡中也可见到这些沉积物。另一方面，在滋养细胞和卵母细胞的线粒体中未检测到沉积物。考虑到昆虫卵母细胞中钙的含量很高（Przel ecka等人[17]），根据可视化钙结合位点的分布，讨论了钙进入卵母细胞的可能途径。
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