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蓖麻幼苗中吡啶辅酶代谢中间体的高效液相色谱分离

High performance liquid chromatography separation of the intermediates of pyridine coenzymes metabolism in Ricinus communis L. seedlings.

作者信息

Martelli P, Bovalini L, Ferri S, Franchi G G

出版信息

Ital J Biochem. 1984 Mar-Apr;33(2):98-105.

PMID:6735686
Abstract

The metabolism of pyridine coenzymes is not yet well clarified in vegetable cells. We propose a HPLC method to separate the compounds involved in the pyridine nucleotides cycle in less than 32 min. The method has been applied to Ricinus communis seedlings. On the basis of the results obtained, the well known "de novo" and "salvage" pathways, demonstrated in other biological tissues for NAD biosynthesis, are likely to occur in this vegetable system.

摘要

吡啶辅酶在植物细胞中的代谢尚未得到充分阐明。我们提出了一种高效液相色谱法,可在不到32分钟的时间内分离参与吡啶核苷酸循环的化合物。该方法已应用于蓖麻幼苗。根据所得结果,在其他生物组织中已证实的用于NAD生物合成的著名的“从头合成”和“补救”途径,很可能也存在于这种植物系统中。

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Ital J Biochem. 1984 Mar-Apr;33(2):98-105.
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