Keller G, Holmes W, Phillips R A
J Cell Physiol. 1984 Jul;120(1):29-35. doi: 10.1002/jcp.1041200105.
Colonies with a unique compact morphology and displaying high replating potential develop in methycellulose cultures when bone marrow cells from hydroxyurea- or 5-fluorouracil-treated mice are cultured in the presence of syngeneic thymocytes. These compact colonies are clonal, originating from a single unit, the compact-colony-forming unit (CFU). Based on replating analysis, at least three groups of compact colonies exist: (1) those that do not replate, (2) those that replate and give only GM colonies, and (3) those that give rise to granulocyte-macrophage (GM), erythroid (E), and mixed-erythroid (MIX/E) colonies. Colonies in the last group have a mean size of 2,060 cells and generate an average of 244 colonies in secondary cultures. Velocity sedimentation studies showed the compact-CFU to be similar in size to the MIX/E-CFU, sedimenting at rates between 3.5 and 7 mm/hour. In addition to giving rise to large numbers of in vitro CFU, compact-CFU are also able to generate day 8 spleen colony-forming units (CFU-S).
当将来自羟基脲或5-氟尿嘧啶处理小鼠的骨髓细胞与同基因胸腺细胞共同培养时，在甲基纤维素培养物中会形成具有独特紧密形态且具有高再接种潜力的集落。这些紧密集落是克隆性的，源自单个单位，即紧密集落形成单位（CFU）。基于再接种分析，至少存在三组紧密集落：（1）不进行再接种的集落，（2）再接种后仅产生粒细胞-巨噬细胞（GM）集落的集落，以及（3）产生粒细胞-巨噬细胞（GM）、红系（E）和混合红系（MIX/E）集落的集落。最后一组集落的平均大小为2060个细胞，在二次培养中平均产生244个集落。速度沉降研究表明，紧密CFU的大小与MIX/E-CFU相似，沉降速率在3.5至7毫米/小时之间。除了能产生大量体外CFU外，紧密CFU还能够产生第8天的脾集落形成单位（CFU-S）。
