Klecker R W, Collins J M
J Chromatogr. 1984 May 11;307(2):361-9. doi: 10.1016/s0378-4347(00)84107-9.
A simple and sensitive method was developed for the separation and quantification of tiazofurin ( TCAR ) in plasma using 2-beta-D- ribofuranosylthiazole -5-carboxamide (iso- TCAR ) or [5-3H] TCAR as an internal standard. The procedure uses C18 extraction columns to clean-up the plasma samples for measurement by high-performance liquid chromatography. A sensitivity of 0.33 microM (0.08 microgram/ml) was easily achieved for 0.5-ml plasma samples. When human whole blood was incubated in vitro with TCAR for 2 h, the plasma concentrations were decreased by 10% (4 degrees C) and 25% (23 degrees C). TCAR could still be measured 22 h after injection of 220 mg/kg into mice. For a two-compartment pharmacokinetic model, the half-lives of TCAR were 18.8 and 412 min.
开发了一种简单灵敏的方法，以2-β-D-呋喃核糖基噻唑-5-甲酰胺（异TCAR）或[5-³H]TCAR作为内标，用于血浆中替唑呋林（TCAR）的分离和定量。该方法使用C18萃取柱对血浆样品进行净化，以通过高效液相色谱法进行测量。对于0.5 ml血浆样品，轻松实现了0.33 μM（0.08 μg/ml）的灵敏度。当人全血在体外与TCAR孵育2小时时，血浆浓度在4℃时降低了10%，在23℃时降低了25%。给小鼠注射220 mg/kg的TCAR后22小时仍可检测到。对于二室药代动力学模型，TCAR的半衰期分别为18.8分钟和412分钟。
