Nocek J E, Polan C E
J Dairy Sci. 1984 May;67(5):1038-42. doi: 10.3168/jds.S0022-0302(84)81403-4.
Nine rations containing three forms (ground hay, .2 to 2 cm; chopped hay, 6 to 10 cm; or all concentrate) and three percents (30, 45, and 60%) of rumen degradable nitrogen were fed to 54 Holstein calves (3 X 3 factorial) between 8 and 20 wk of age. Rumen fluid samples were obtained at 8, 11, 14, 17, and 20 wk of age and plasma at 14 and 20 wk of age. Rumen fluid acetate concentration was greatest for ground hay and lowest for concentrate; propionate was greatest for concentrate and lowest for calves fed ground hay. Calves fed concentrate had highest valerate and total volatile fatty acid concentration, whereas lactate concentration and pH were least for concentrate. Ruminal ammonia nitrogen increased with increasing ruminally degradable protein feedstuffs in the ration. Plasma acetate was highest for chopped hay and lowest for concentrate, whereas calves fed concentrate and ground hay were comparable with higher propionate and glucose concentrations than calves fed chopped hay. Plasma urea nitrogen was highest for calves fed 60% ruminally degradable protein.
将含有三种形式(粉碎干草,0.2至2厘米;切碎干草,6至10厘米;或全精料)和三种瘤胃可降解氮比例(30%、45%和60%)的九种日粮饲喂给54头荷斯坦犊牛(3×3析因设计),犊牛年龄在8至20周龄之间。在8、11、14、17和20周龄时采集瘤胃液样本,在14和20周龄时采集血浆样本。粉碎干草组的瘤胃液乙酸盐浓度最高,精料组最低;丙酸盐浓度精料组最高,饲喂粉碎干草的犊牛最低。饲喂精料的犊牛戊酸盐和总挥发性脂肪酸浓度最高,而精料组的乳酸浓度和pH值最低。日粮中瘤胃可降解蛋白质饲料增加时,瘤胃氨氮升高。切碎干草组的血浆乙酸盐最高,精料组最低,而饲喂精料和粉碎干草的犊牛丙酸盐和葡萄糖浓度高于饲喂切碎干草的犊牛。饲喂60%瘤胃可降解蛋白质的犊牛血浆尿素氮最高。