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三磷酸腺苷与大变形虫收缩泡的自主性

ATP and the autonomy of the contractile vacuole in Amoeba proteus.

作者信息

Pothier F, Couillard P, Forget J

出版信息

J Exp Zool. 1984 May;230(2):211-8. doi: 10.1002/jez.1402300206.

DOI:10.1002/jez.1402300206
PMID:6736893
Abstract

Contractile vacuole function in amoebae treated with immobilizing (5 mM) and nonimmobilizing (0.125 mM) concentrations of ATP has been studied. In ATP-immobilized amoebae, most vacuolar parameters are accelerated, especially the rate of output which passes from 30 to 70 micron3/sec. This favors the concept of an autonomous vacuole, fully functional in the absence of any bulk contribution to it from remote points of the cell. A lower concentration of ATP (0.125 mM), which does not inhibit movement, causes a still greater acceleration of vacuolar function. Work is in progress to elucidate the site and mode of action of exogenous ATP on Amoeba.

摘要

研究了用固定浓度(5 mM)和非固定浓度(0.125 mM)的ATP处理的变形虫中收缩泡的功能。在ATP固定的变形虫中,大多数液泡参数加快,尤其是输出速率从30立方微米/秒增加到70立方微米/秒。这支持了自主液泡的概念,即在没有细胞远端任何大量贡献的情况下,液泡仍能充分发挥功能。较低浓度的ATP(0.125 mM)不抑制运动,但会使液泡功能加速得更明显。目前正在进行研究,以阐明外源ATP对变形虫的作用位点和作用方式。

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