一种具有高空间和时间分辨率的自动脉冲激光微荧光计。

An automatic pulsed laser microfluorometer with high spatial and temporal resolution.

作者信息

Docchio F, Ramponi R, Sacchi C A, Bottiroli G, Freitas I

出版信息

J Microsc. 1984 May;134(Pt 2):151-60. doi: 10.1111/j.1365-2818.1984.tb02504.x.

DOI:10.1111/j.1365-2818.1984.tb02504.x

Abstract

The paper describes an automatic pulsed laser microfluorometer with high spatial and temporal resolution, developed in our laboratories. The instrument consists of: (i) a nitrogen-laser-pumped dye-laser for the excitation of the fluorescence, (ii) a microscope with additional optics to focus the excitation beam on the sample and to collect the fluorescence, (iii) filters or monochromators to select the output wavelength, (iv) a fast photomultiplier tube to detect the signal, and (v) a dual time-scale microprocessor-controlled signal averager for the acquisition and processing of the signal. Examples are given that show the potential of the time-resolved fluorescence microscopy in studying, quantitatively and qualitatively, the properties of fluorescent molecules.

摘要

本文描述了一种在我们实验室研发的具有高空间和时间分辨率的自动脉冲激光微荧光计。该仪器包括：（i）一台由氮激光泵浦的染料激光器，用于激发荧光；（ii）一台配有附加光学器件的显微镜，用于将激发光束聚焦在样品上并收集荧光；（iii）用于选择输出波长的滤光片或单色仪；（iv）一个快速光电倍增管，用于检测信号；以及（v）一个双时间尺度微处理器控制的信号平均器，用于信号的采集和处理。文中给出的示例展示了时间分辨荧光显微镜在定量和定性研究荧光分子特性方面的潜力。