Piper H M, Hütter J F, Spieckermann P G
Life Sci. 1984 Jul 9;35(2):127-34. doi: 10.1016/0024-3205(84)90131-0.
Cultured adult cardiac myocytes were exposed to anoxia under substrate-free conditions. When compared to the metabolic changes in the oxygen deficient organ, those in the anoxic cell culture proceed in a similar, yet prolonged manner. Release of cytosolic enzymes starts with minor energetic disturbances and proceeds in close correlation to the actual ATP decay. Below 2 mumol ATP/gww, an increasing number of cells becomes irreversibly damaged, but above, 30 min reoxygenation leads to extensive recovery of the whole preparation. The results indicate that leakage of cytosolic enzymes during the early stage of anoxia is due to a gradual protein release from the individual cells, related to reversible membrane alterations.
在无底物条件下，将培养的成年心肌细胞置于缺氧环境中。与缺氧器官中的代谢变化相比，缺氧细胞培养中的代谢变化以相似但更持久的方式进行。胞质酶的释放始于轻微的能量紊乱，并与实际的ATP衰减密切相关。当ATP含量低于2微摩尔/克湿重时，越来越多的细胞会受到不可逆损伤，但高于此值时，30分钟的复氧可使整个制剂得到广泛恢复。结果表明，缺氧早期胞质酶的泄漏是由于单个细胞中蛋白质的逐渐释放，这与可逆的膜改变有关。
