Szybiak U, Bouley J P, Fritsch C
Nucleic Acids Res. 1978 Jun;5(6):1821-31. doi: 10.1093/nar/5.6.1821.
On centrifugation in a CsCl density gradient the three tymoviruses, eggplant mosaic, wild cucumber mosaic, and okra mosaic, separate into a major bottom component and several less dense minor components. The RNA of the top component is composed of about 90% tRNA and 10% of an approximately 250 000 dalton messenger RNA. The latter induced the synthesis of coat protein when translated in wheat-germ and rabbit-reticulocyte cell-free systems.
在氯化铯密度梯度中进行离心时，三种芜菁黄花叶病毒，即茄子花叶病毒、野生黄瓜花叶病毒和秋葵花叶病毒，分离成一个主要的底部成分和几个密度较小的次要成分。顶部成分的RNA约由90%的tRNA和10%的一种约250000道尔顿的信使RNA组成。当后者在小麦胚芽和兔网织红细胞无细胞系统中进行翻译时，可诱导外壳蛋白的合成。
Zotero 插件
